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The mice were anesthetized with 300 mg/kg chloral hydrate and were intratracheally injected with 3 mg/kg BLM (Meilun Biotechnology Co., Ltd., Dalian, China) in 100 μl of saline via tracheostomy to induce pulmonary fibrosis

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Complete regular lab work to monitor IGF-1, hormone levels, and metabolic markers, adjusting protocols as needed

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